dapi stain solution (Sangon Biotech)
86
Structured Review
Sangon Biotech
dapi stain solution
Dapi Stain Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+dapi/dapi+solution+staining/pmc13138055-43-0-4
Average 86 stars, based on 1 article reviews
Dapi Stain Solution, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staining+dapi/dapi+solution+staining/pmc13138055-43-0-4
Average 86 stars, based on 1 article reviews
dapi stain solution - by Bioz Stars,
2026-09
86/100 stars
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Staining:Article Title: An Immune Microenvironment-Integrated Intestinal-on-a-Chip Model for Investigating Immunopathogenesis in Inflammatory Bowel Disease. Article Snippet: .. Finally, add Article Title: The functional landscape of alternative splicing in hematopoietic lineage commitment Article Snippet: The cells were loaded on poly-lysine treated slides and fixed with 4% paraformaldehyde at RT for 15 min. After washing with PBS for three times, cells were permeabilized by 0.1% TritonX-100 at RT for 15 min followed by blocking with 10% FBS in PBS at RT for 1 h. Cell staining was performed using primary antibodies including Rabbit anti-TGN46 (1:300, Abcam, ab50595), Mouse anti-GM130 (1:200, BD, #610822), Mouse anti-Cl-MPR (1:200, Bio-Rad, #MEM-2), Rabbit anti-ZFPL1 (1:200, Invitrogen, #PA5-53593), Rabbit anti-TBC1D23 (1:50, Sigma, #HPA038152) or Mouse anti-RanBP2 (1:1000) at 4 °C overnight. .. The cells were washed again with PBS for three times and incubated with secondary antibodies, including FITC affinipure goat anti-Mouse IgG antibody (1:2000, Jackson ImmunoResearch), FITC affinipure goat anti-Rabbit IgG antibody (1:2000, Jackson ImmunoResearch, #115-095-003) or Alexa Fluor 647 affinipure goat anti- Mouse IgG antibody (1:2000, Jackson ImmunoResearch, #115-605-003) at 37 °C for 1 h. The nuclei were stained with Article Title: Harnessing the yeast nucleus for the efficient production of valuable monoterpenes. Article Snippet: Subcellular engineering emerges as a pivotal strategy to amplify terpene production in yeast cell factories.. The yeast nucleus, which shares acetyl-CoA pool with the cytosol and maintains nuclear membrane integrity during mitosis, offers unique compartmentalization capabilities.. Despite these attributes, there is a notable absence of research harnessing the potential of the yeast nucleus for subcellular engineering. Article Title: The functional landscape of alternative splicing in hematopoietic lineage commitment. Article Snippet: After washing with PBS for three times, cells were permeabilized by 0.1% TritonX-100 at RT for 15min followed by blocking with 10% FBS in PBS at RT for 1 h. Cell staining was performed using primary antibodies including Rabbit anti-TGN46 (1:300, Abcam, ab50595), Mouse antiGM130 (1:200, BD, #610822), Mouse anti-Cl-MPR (1:200, Bio-Rad, #MEM-2), Rabbit anti-ZFPL1 (1:200, Invitrogen, #PA5-53593), Rabbit anti-TBC1D23 (1:50, Sigma, #HPA038152) or Mouse anti-RanBP2 (1:1000) at 4 °C overnight. .. The cells were washed again with PBS for three times and incubated with secondary antibodies, including FITC affinipure goat anti-Mouse IgG antibody (1:2000, Jackson ImmunoResearch), FITC affinipure goat anti-Rabbit IgG antibody (1:2000, Jackson ImmunoResearch, #115-095-003) or Alexa Fluor 647 affinipure goat anti- Mouse IgG antibody (1:2000, Jackson ImmunoResearch, #115-605-003) at 37 °C for 1 h. The nuclei were stained with Article Title: Nrf2 overexpression reprograms neural stem cell fate: promoting neuronal differentiation and functional recovery post-ischemic stroke via suppression of the ROS/NF-κB axis Article Snippet: The secondary antibody Alexa Fluor 555 conjugated Donkey anti-rabbit (1:500; Beyotime Biotechnology), was incubated for 60 min at room temperature in the dark. .. Nuclei were stained with Article Title: Screening and Functional Validation of Host Interacting Proteins for the Key Invasion Protein LRR5 of Mycoplasma bovis. Article Snippet: Mycoplasma bovis induces a range of inflammatory conditions in cattle, causing substantial economic losses to the global cattle industry.. The pathogen employs adhesion and invasion mechanisms to evade host immune responses, enabling long-term infection.. Therefore, identifying invasion-related virulence factors and their potential host receptors is crucial for elucidating the pathogenic mechanisms of M. bovis and developing targeted control strategies. Article Title: An Immune Microenvironment-Integrated Intestinal-on-a-Chip Model for Investigating Immunopathogenesis in Inflammatory Bowel Disease Article Snippet: .. Finally, add Article Title: The missense mutation Y65C in PQBP1 causes microcephaly and cognitive deficits through a combination of partial loss-of-function and gain-of-function effects. Article Snippet: The following secondary antibodies diluted at 1:200 were used for immunofluorescence: Alexa Fluor 555 goat anti-rabbit IgG (ab150078, Abcam);Alexa Fluor 488goat anti-rabbit IgG (ab150081, Abcam);Alexa Fluor 488 goat anti-mouse IgG (ab150117, Abcam); Alexa Fluor 555 goat anti-mouse IgG (ab150118, Abcam); Alexa Fluor 647 goat anti-rabbit IgG (ab150079, Abcam); Alexa Fluor 647 goat anti-mouse IgG (ab150115, Abcam); Alexa Fluor 488 goat anti- Chicken IgG (ab150169, Abcam); Alexa Fluor 555 goat anti-Rat IgG (ab150158, Abcam); Alexa Fluor 488 goat anti-Rat IgG (ab150157, Abcam). .. Incubation:Article Title: The functional landscape of alternative splicing in hematopoietic lineage commitment Article Snippet: The cells were loaded on poly-lysine treated slides and fixed with 4% paraformaldehyde at RT for 15 min. After washing with PBS for three times, cells were permeabilized by 0.1% TritonX-100 at RT for 15 min followed by blocking with 10% FBS in PBS at RT for 1 h. Cell staining was performed using primary antibodies including Rabbit anti-TGN46 (1:300, Abcam, ab50595), Mouse anti-GM130 (1:200, BD, #610822), Mouse anti-Cl-MPR (1:200, Bio-Rad, #MEM-2), Rabbit anti-ZFPL1 (1:200, Invitrogen, #PA5-53593), Rabbit anti-TBC1D23 (1:50, Sigma, #HPA038152) or Mouse anti-RanBP2 (1:1000) at 4 °C overnight. .. The cells were washed again with PBS for three times and incubated with secondary antibodies, including FITC affinipure goat anti-Mouse IgG antibody (1:2000, Jackson ImmunoResearch), FITC affinipure goat anti-Rabbit IgG antibody (1:2000, Jackson ImmunoResearch, #115-095-003) or Alexa Fluor 647 affinipure goat anti- Mouse IgG antibody (1:2000, Jackson ImmunoResearch, #115-605-003) at 37 °C for 1 h. The nuclei were stained with Article Title: The functional landscape of alternative splicing in hematopoietic lineage commitment. Article Snippet: After washing with PBS for three times, cells were permeabilized by 0.1% TritonX-100 at RT for 15min followed by blocking with 10% FBS in PBS at RT for 1 h. Cell staining was performed using primary antibodies including Rabbit anti-TGN46 (1:300, Abcam, ab50595), Mouse antiGM130 (1:200, BD, #610822), Mouse anti-Cl-MPR (1:200, Bio-Rad, #MEM-2), Rabbit anti-ZFPL1 (1:200, Invitrogen, #PA5-53593), Rabbit anti-TBC1D23 (1:50, Sigma, #HPA038152) or Mouse anti-RanBP2 (1:1000) at 4 °C overnight. .. The cells were washed again with PBS for three times and incubated with secondary antibodies, including FITC affinipure goat anti-Mouse IgG antibody (1:2000, Jackson ImmunoResearch), FITC affinipure goat anti-Rabbit IgG antibody (1:2000, Jackson ImmunoResearch, #115-095-003) or Alexa Fluor 647 affinipure goat anti- Mouse IgG antibody (1:2000, Jackson ImmunoResearch, #115-605-003) at 37 °C for 1 h. The nuclei were stained with |
